High-level expression of soluble recombinant RNase P protein from Escherichia coli.

نویسندگان

  • R Rivera-León
  • C J Green
  • B S Vold
چکیده

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 177 9  شماره 

صفحات  -

تاریخ انتشار 1995